Applications
Gene editing / CRISPR-Cas
CRISPR-Cas stands out as the latest and most widely used genome editing technology, offering a significant reduction in time needed to create new models for studying gene function in eukaryotes. Controlling Cas expression via the Tet technology presents an attractive solution to prevent off-target effects and to enable precise, tissue- and time-specific gene editing in animal models and iPS cells.
Recent Literature:
Jurlina et al. (2022) A Tet-Inducible CRISPR Platform for High-Fidelity Editing of Human Pluripotent Stem Cells: Genes 2022 Dec 14;13(12) 2363
Dow et al. (2015) Inducible in vivo genome editing with CRISPR-Cas9: Nat Biotechnology 2015 Apr;33(4): 390-393
