Technology
Science and Technology
The Tet Technology provides efficient, precise and reversible control over both timing and level of gene expression in eukaryotic cells. Its outstanding specificity and the well-studied chemical and physiological properties of the inducing agents have made the TET System the most widely applied inducible gene expression system.
The Tet Technology has been successfully applied in numerous tissue culture systems as well as in various organisms such as yeasts, protozoans, insects, amphibia, plants and mammals, particularly in mice. Besides facilitating physiological studies at the cellular level, the Tet Technology also permits detailed analyses of complex biological processes like behavior, development and disease. Typical applications are listed here.
Tet-On 3G
No DOX
The Tet-On 3G system, combines an optimized tetracycline-dependent reverse transactivator with an improved responsive promoter. The Tet-On 3G transactivator, activates expression of responsive genes in the presence of the inducing agent (e.g. doxycycline, Dox). It consists of a synthetic, optimized Tet repressor moiety and a minimal VP16 activation domain, conferring significantly increased sensitivity to Dox compared to previous versions. This increased sensitivity is particularly advantageous for in vivo studies in tissues where high Dox concentrations are difficult to attain (e.g., brain).
+ DOX
The newly designed PTRE3G promoter confers tight, virtually background-free expression in the non-induced state and high expression levels after addition of the inducing agent. Thus, the Tet-On 3G system provides an excellent dynamic range for regulating gene expression without interfering with host cell physiology.
Applications
Stem cells (eg. iPS cells)
The discovery that differentiated tissue cells can transform into embryonic-like stem cells, known as inducible pluripotent stem cells (iPS), marked a pivotal scientific breakthrough, earning the Nobel Prize in 2012. Induced pluripotent stem cells provide an exciting alternative preventing ethical issues associated with embryonic…
Gene editing / CRISPR-Cas
CRISPR-Cas stands out as the latest and most widely used genome editing technology, offering a significant reduction in time needed to create new models for studying gene function in eukaryotes. Controlling Cas expression via the Tet technology presents an attractive solution to prevent off-target effects and to prevent…
Mouse/Animal Models
Conventional transgenic and knockout animals, while widely used for research and disease modelling, suffer from limitations such as embryonic lethality and irreversible gene deletions. The Tet Technology addresses these challenges by enabling tight, time- and tissue-specific, reversible control of gene expression in vivo. Tet…
Commercial applications
The Tet Technology is utilized by various commercial entities, however, these applications often have no public accessibility. Typical applications include: Manufacturing: The Tet Technology is perfectly suited to generate cell lines for studies of toxic gene products. Cells can be expanded to high density before activating…
